Test Code URGS Urine Gram Stain
Specimen Requirements
1. Clean Catch Midstream Specimen
a. Thoroughly clean the glans (male) or urethral area (female) with
soap and water.
b. Rinse area with wet gauze pads.
c. While holding the foreskin (male) or labia apart (female), have
patient begin voiding.
d. After several mL of urine have passed, collect midstream portion
into a sterile screw-cap container without stopping urine flow.
e. Transport specimen to laboratory without delay.
2. Catheterized Specimen
a. Thoroughly clean urethral area with soap and water.
b. Rinse area with wet gauze squares.
c. Aseptically insert catheter into bladder.
d. Allow 15 mL of urine to pass before collecting urine in a
sterile screw-cap container.
e. Alternatively urine can be collected by sterile syringe and
needle aspiration using the collection port of an indwelling
catheter.
f. Transport specimen to laboratory without delay.
g. Foley catheter tips should not be submitted or accepted for
culture because they will invariably be contaminated with skin
flora and quantitation is not possible.
SPECIMEN PROCESSING and HANDLING
Submit urine specimen in grey-top urine culture collection tube, if only culture requested.
TRANSPORT TEMPERATURE
Refrigerate any specimen that cannot be submitted to the lab within two hours of collection
PERFORMING LAB
WKMC