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HelpTest Code FPGT Phenosense Combination HIV Drug Resistance Assay
Reporting Name
Phenosense GTPerforming Laboratory
Monogram Biosciences, IncSpecimen Type
Plasma EDTAOrdering Guidance
This procedure should be used for patients with documented HIV-1 infection and viral loads =500 copies/mL.
Specimen Required
Specimen Type: Plasma
Container/Tube: Lavender-top (EDTA) or plasma preparation tube (PPT)
Specimen Volume: 3 mL
Collection Instructions: Draw blood in either PPT (pearl top) or lavender-top (EDTA) tubes. Centrifuge specimen within six hours of collection. Remove plasma from cells immediately, and transfer specimen to a screw-capped, plastic vial. Freeze 3 mL of PPT plasma or EDTA plasma immediately, send specimen frozen.
To avoid delays in turnaround time when requesting multiple tests, please submit separate frozen specimens for each test requested.
RECOMMENDED:
1.    Patient's most recent viral load.
2.    Viral load collection date.
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NOTE:Â 1. Intended for use only for patients with viral loads greater than or equal to 500 copies/mL. For best results, viral loads should be confirmed within 2 weeks prior to submission for testing at Monogram.
           2. Patient samples submitted <30 days apart are considered duplicate and will be cancelled.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 14 days |
Reference Values
A final report will be attached in MayoAccess.
Day(s) Performed
Sunday through Saturday
CPT Code Information
87900/Infectious agent drug susceptibility phenotype prediction
87901/Infectious agent genotype analysis by nucleic acid; reverse transcriptase and protease
87903/Infectious agent phenotype analysis by nucleic acid with drug resistance tissue culture analysis; first through 10 drugs tested
87904/x12 Each additional drug tested
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FPGT | Phenosense GT | Not Provided |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| Z1043 | Phenosense GT | Not Provided |
Report Available
18 to 26 daysReject Due To
| Other | PPT(TM) not centrifuged; quantity not sufficient for analysis; heparinized plasma; nonfrozen specimen; specimen exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube |
Method Name
Phenotype: polymerase chain reaction (PCR) amplification and viral culture; genotype: polymerase chain reaction (PCR) and DNA sequencing.
Useful For
Determines viral phenotype and genotype resistance to three classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), nonnucleoside reverse transcriptase inhibitors (NNRTI), and protease inhibitors (PI). Viral replication capacity is also included.