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Test Code C2 C2 Complement, Functional, with Reflex, Serum

Important Note

GEL/SST SPECIMENS NOT ACCEPTED!

 

Per Mayo, the most important thing about processing Complement Testing is keep the specimen COLD (< 10⁰C)!  Ice packs can be used to collect, and the blood should clot on ice or refrigerated. Ice packs warm up quickly so you must monitor and change the ice packs when they warm up if using ice packs to clot. Once the blood has clotted, it should be spun down and FROZEN IMMEDIATELY. 

Reporting Name

C2 Complement,Functional,w/Reflex,S

Useful For

Investigation of a patient with a low (absent) hemolytic complement, with reflex testing to C3 and C4, if appropriate

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
C4 Complement C4, S Yes No
C3 Complement C3, S Yes No

Testing Algorithm

If the C2 result is less than 15 U/mL, then complement C3 and C4 will be performed at an additional charge.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Ordering Guidance


This test is for assessment of complement C2 and includes assessment of C3 and C4 as reflex testing. Unless a deficiency has already been identified, initial assessment should begin with the total complement assay (COM / Complement, Total, Serum), which is a screen for suspected complement deficiencies and should be performed before ordering individual complement component assays. A deficiency of an individual component of the complement cascade will result in an undetectable total complement level.



Specimen Required


Patient Preparation: Fasting preferred but not required

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge and aliquot serum into plastic vial.

3. Immediately freeze specimen.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Frozen 21 days

Reference Values

25-47 U/mL

Day(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86161

86160 x 2 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
C2 C2 Complement,Functional,w/Reflex,S 93977-7

 

Result ID Test Result Name Result LOINC Value
C2FX C2 Complement,Functional,S 93977-7
INT53 Interpretation 69048-7

Report Available

1 to 3 days

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Method Name

Automated Liposome Lysis Assay